After study 2, a separate group of historical controls (Table 2, group B) was acquired, and these macaques showed pulmonary lesions similar to those seen in control B. In all of the control macaques (n = 8), the lesions present in the liver…
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S2 for immunization details). (D) The purity of TMCP2 determined by high-performance reverse-phase separation. (E) Positive electrospray ionization time-of-flight mass spectrometry (TOF MS ES+) of purified TMCP2. The P. aeruginosa PAO1 WT strain was kindly provided by S. Lory (Harvard University) (26). The other bacterial strains and plasmids are listed in Table S1A. PAO1 and its derivatives were grown for ∼16 h on lysogeny broth (LB) agar or liquid… We also examined EMCV-induced cell death in HEK293T cells (Fig. 2C). WT HEK293T cells were very sensitive to EMCV-induced cell death at all MOIs, with ≤5% survival of cells at doses as low as an MOI of 0.001. 16S rRNA amplicon sequencing of fecal microbiota.Total DNA was extracted from all fecal samples (∼200 mg) using the QIAamp DNA stool minikit (Qiagen, Crawley, United Kingdom) according to the manufacturer's instructions apart from adding a… Fundamental electrophysiological experiments on nonhuman primates have demonstrated that midbrain dopamine cells fire both during anticipation of uncertain rewards and at the time of unexpected reward delivery (1, 11).
S2 for immunization details). (D) The purity of TMCP2 determined by high-performance reverse-phase separation. (E) Positive electrospray ionization time-of-flight mass spectrometry (TOF MS ES+) of purified TMCP2.
